Gene variants/mutagenesis
The combination of GENEART’s strong expertise in de novo gene synthesis and the reuse of existing DNA constructs allows us to synthesize complex gene variants more cost-effectiv. This strategy allows the inclusion of several mutations at the same time, while maintaining the high GENEART quality standards.
Functional protein analysis often requires the generation of several variants of the wildtype gene. An alanine or cysteine scan, glycosylation modification and deletion experiments are just a few examples. To date, this has required tedious mutagenesis bench work. PCR-based protocols involve the screening of many clones to identify non-wildtype and correct mutants and are prone to insert and vector integrity errors. The process is time-consuming and can become expensive, not only with regards to the sequencing costs.
Save your valuable time and money for the really important experiments and profit from GENEART's extended service. Our expertise in de novo gene synthesis allows us to generate gene variants with complex combinations of extensive substitutions, insertions and deletions, with delivery times starting at just two weeks.
All gene variants are subcloned into the vector of your choice with a100% sequence guarantee and delivered as 10 µg of plasmid DNA.
Please use our online request form to obtain a quote for your specific project.
Print