Antibody production by genetic immunization
Traditional methods of antibody production rely on proteins or peptides which are essential for animal immunization. The procedure for purification of the relevant protein or peptide antigens is laborious, time-consuming and often leads to partly degraded or contaminated antigens.
Genetic immunization is an attractive alternative for antibody production. It delivers antigen-coding plasmid DNA into the animal. The animal's cells produce the protein from the expression vectors, which stimulates the animal's immune system to produce antibodies against that particular protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA can be produced more quickly, cheaply and flexibly than protein. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
The most important advantages of antibody production by genetic immunization are as follows:
- It is faster because there is no need for purified protein or peptide, just a DNA sequence file
- It saves time and money
- It generates antibodies with high affinity and specificity
- It generates antibodies against: native protein structures, rarely expressed polypeptides, toxic or self-antigens
> Monoclonal antibody from DNA sequence file
GENEART offers antibody production in cooperation with GENOVAC.
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