Directed Evolution
In vitro molecular evolution strategies are perhaps the most efficient methods for creating proteins and regulatory sequences with improved or novel properties. Different library types for a variety of different approaches are available. Please select one of the choices in the list below to learn more about the specific library types.
Degenerated Libraries (up to 1011 variants)
degeneration of multiple codons
Site-Saturation Mutagenesis (up to 20 variants)
degeneration of one single codon
Sequential Permutation Libraries (# of codons x 20 variants)
successive site-saturation mutagenesis
Controlled Randomization (up to 1011 variants)
unbiased random substitutions with defined frequency
SH3 Domain Phage Display Library
cloned cDNA of all known human SH3 domains
GENEART has successfully resolved recent shortcomings by constructing libraries of customers' genes or regulatory sequences synthetically and from scratch. Our excellent expertise in gene synthesis allows us to generate synthetic libraries on a very rational basis with highest degree of correctness in the non-random, constant framework region and an equal distribution of nucleotides in the partially degenerated or fully randomized parts.
Supported by our GeneOptimizer® assisted sequence design, synthetic libraries help you to obtain a complete representation of all desired variants while avoiding unwanted mutants. This approach dramatically reduces the size of your "haystack" and cuts down screening time and costs while increasing your experimental success.
GENEART technology facilitates the generation and broadens the diversity of your gene library. De novo gene synthesis enables convenient construction of rare allelic variations and combinations thereof. Automated synthesis of the library and the high GENEART quality standard ensures the production of your screening source with short delivery times and a value you can count on.
Please refer here for a FAQ about GENEART's services for directed evolution.
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