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Site-directed Mutagenesis: ITERATE Advanced

GENEART’s site-directed mutagenesis service ITERATE Advanced introduces single or multiple mutations (substitutions, insertions, deletions or truncations) into existing DNA sequences within shortest turnaround times.

examplary site directed mutagenesis with multiple Ala-substitutions into existing DNA sequence


Applications

  • Study of active sites, structure - function relationships, nucleic acid - protein interactions etc.
  • Construction of fusion proteins and tagged proteins
  • Construction of knockout constructs
  • Generation of alternative splice forms
  • Test of functional elements (promoter regions, terminator sequences etc.)
  • Alanine scans
  • Example ITERATE Advanced

Advantages

  • Mutations at any site
  • No quick change involved:
                  - Insert size can be as large as 6-8 kb
                  - No mutations in the vector backbone
                  - One-step-mutations at distant sites possible (faster turn around)
  • Ultra fast turn around times
  • Largest capacities worldwide
  • Long term storage of template constructs
  • Full sequence verification of insert DNA
  • Most competitive pricing

Deliverables

Cat. No Product name Deliverables
839951 ITERATE Advanced
(Site-directed mutagenesis)
5 µg of plasmid DNA

Quality control

All production processes are ISO 9001:2008 certified.
All constructs are completely sequence verified; comprehensive documentation provided.
Please use our online request form to obtain a quote for your specific project.


* offer is valid for:

  • ≤ 5 constructs: subcloning into GENEART standard vector
  • ≥ 6 constructs: subcloning into customer vector; (only commercially available standard vectors); for 100.- cloning fee per started package of 10 constructs
  • genes up to 2 kb
  • E. coli only

Please use our online customer portal to obtain a quote for your specific project.

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