Site-Saturation Mutagenesis
(up to 20 variants)
degeneration of one single codon
The Problem
Once a specific amino acid position within a polypeptide has been identified to be critical for the protein's function, it is of interest to identify the ideal amino acid for this position.
The Solution
Site-saturation mutagenesis allows the substitution of specific sites against all 20 possible amino acids at once. This is much more cost effective than generating these constructs separately.
Deliverables
All gene variants are subcloned into the customer's vector and delivered as 10 µg of plasmid DNA.
Depending on the details of the customer's screening assay, site-saturated constructs can be delivered in two different formats:
- mixed (small library containing all 20 variants)
- separate (each of the 19 substitution variants will be delivered separately)
Quality control
A mixed site-saturated library will be bulk sequenced.
Separated variants will be 100 % sequence verified each.
Fill out our online quotation form to get a quote for your specific project.
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