How can I order?  

Use our DNA sequencing orderform and fill in your personal data including the e-mail address. Select the kind of sequencing service, fill in the sample names and send the probes and order sheet together to the following adress:  

GENEART AG Sequencing Service
Josef-Engert-Str. 11
D-93053 Regensburg  

The results will be sent within two business days by e-mail.  

What does "ready to use" mean?  

The probe contains the right amount of template, primer and water in a volume of 8µl.  

How much Template do I have to use?  

For plasmids up to 6kb (vector + insert) 250ng of DNA are enough for one read. 10pMol of oligo are needed for one standard reaction.   For sequencing PCR-Products we have different protocols:
Per 100bp length of a PCR-product, we will need about 10-20ng of purified products. (e.g. for a product length of 350bp we need between 35ng - 75ng PCR-product and 10pMol Oligo per reaction).  
For sequencing viral-, Bac-, or Bacmid-DNA please send 10µg Template in a concentration of 500ng/µl and the oligos in a concentration of 100 pMol/µl  

Offers GENEART template purification?  

Yes! For PCR-Products we use columns for purification
 

Are there primers for standard-vectors available?

Yes!  We have following primer for free of charge:  M13 (-21), M13rev, pQE-Prom, pQErev, 5’AOX, 3’AOX, pGEX5’, pGEX3’, pLNCX-for, pLNCX-rev, N-CMV-30, C-CMV-24, T7, T7term, SP6, T3, Rv3, GL2, BGHrev. There is also the possibility to get customized primers for primerwalking, different vectors, etc.   
 

What does "rerun of order" mean?  

The customers get an ordernumber when the sequences are being prepared. If some probes failed the first time, the customers have the possibility to have the probes repeated with different sequencing protocolls. The customers just have to send new DNA/primers and give us the ordernumber that should be repeated.

Does GENEART do Bac-End Sequencing?  

Yes. For this purpose please send DNA (minimum 10µg) and primer (100 pMol) separately.    

What kind of template information is necessary?  

The more information we can get,  the better the results will be: Has the templete a high GC-content, repetitive elements, homopolymere (A/T-runs)? We can use different sequencing protocolls to get the best results.    

Repeats, AT-rich sequence:
standard sequencing protocoll                                                modified protocoll