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SH3 library


All SH3-Domain Phager:

Detect specific human SH3 domain interactions for your protein of interest.

Principle of Phage Display: Target protein coupled onto paramagnetic band is exposed to library of phages displaying all human SH3 domains. Unbound phages are washed away. Specifically bound phage is eluted and amplified. Individual clones are isolated and sequenced.

Our ready-to-use assay provides:

  • Fast and unbiased detection of binding targets from a pool of more than 300 human SH3 domains
  • Entire selection of individual SH3 domains which bind your specific ligand
  • High specificity guaranteed due to lack of spurious interference from other non-target proteins
  • Applicable to any target protein
  • Easy association of selected SH3 domains to corresponding protein via accession number

Our All SH3-Domain Phager is the only assay available for an easy and simultaneous screening of the complete human SH3 proteome for interaction partners of your protein.

 

References:

  • Tapio Kesti, Anja Ruppelt, Jing-Huan Wang, Michael Liss, Ralf Wagner, Kjetil Taskén and Kalle Saksela
    Reciprocal Regulation of SH3 and SH2 Domain Binding via Tyrosine Phosphorylation of a Common Site in CD3
    The Journal of Immunology, 2007, 179: 878-885.

  • Kärkkäinen S, Hiipakka M, Wang JH, Kleino I, Vaha-Jaakkola M, Renkema GH, Liss M, Wagner R, Saksela K. (2006)
    Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome.
    EMBO Rep. 2006 Feb;7(2):186-91.
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