Europe & Asia
phone: +49 (0)941 942 76-100
fax:        +49 (0)941 942 76-780
US & Canada
phone:+1-877-720-GENE (4363)
fax:       +1-416-233-0854

Gene construction

Gene construction includes bioinformatics, oligosynthesis, gene assembly, cloning, sequencing and the final documentation

Sequence design, gene analysis and  
gene optimization (optional and for free) are performed by a team of highly skilled scientists.1)

 

Our genes are assembled from synthetic oligonucleotides produced in-house with the highest level of sequence accuracy.

GeneAssembler® * allows high-throughput gene assembly on an industrial scale and is the only industrial gene processing platform.2)

 

All genes will be cloned into one of our standard cloning vectors. 3),5)
 

All genes will be checked by in-house sequence verification. Only 100% correct sequences will be shipped.4)

 

Final quality control is performed by a separate QM unit. A detailed synthesis report including plasmid map (gb files), sequence alignment and restriction map will be provided.6)

 1) Gene optimization and analysis are based on our proprietary   
     GeneOptimizer® software.

2) The DNA molecules are produced via Polymerase Chain Reaction under
    license (F. Hoffmann-La Roche Ltd).

3) GENEART reserves the right to deliver sequences in any appropriate cloning
    vector. No responsibility can be accepted for the presence or absence of
    restriction sites, antibiotic markers, promoters or other sequence elements in
    the vector backbone.

4) GENEART guarantees a 100% match between the synthesized sequence and
    the customer sequence ordered and confirmed.

5) As some sequences turn out to be toxic and/or genetically unstable in a small
    number of cases, the quoted turnaround times only apply to sequences which
    are non-toxic for and genetically stable in E. coli. In exceptional cases, a
    reduced quantity of toxic fragments may be supplied, in cloned fragments or as
    quality-controlled linear DNA.

6) The GENEART gene synthesis process is fully DIN EN ISO 9001:2008 
    certified. Our internal quality management system covers gene optimization,
    gene synthesis, DNA sequencing and sales, ensuring reliability and
    reproducibility for our customers.

 

* Gene Assembler is a registered german trade mark.

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