GeneOptimizer^® – unique multiparameter optimization

Our proprietary industry-preferred optimization algorithm considers all relevant optimization parameters in one single operation.

GeneOptimizer^® software tool

Taking into account the most important parameters in parallel, the software generates a total of up to 500,000 optimized variants of your target sequence in an evolutionary approach and selects the one that suits your requirements best. Some customers have reported up to a 100-fold increase in expression yields compared to the original gene sequence.

Proven increase in protein yield through mRNA stabilization and utilization of translational efficacy:

Reference: Graf M. et al (2000), J Virol 2000 Nov; 74(22): 10822-6

We have been extremely thorough in our development of this software over the past few years and are constantly updating its features based on new development .

GeneOptimizer^® benefits

Database cloning
Removal of introns
Knockout of cryptic splice sites and RNA destabilizing sequence elements
Increased RNA stability
Adaptation of codon usage
Extensive mutagenesis
Flexible combination of functional domains
Introduction of restriction sites
Epitope shuffling
Consideration of immune modulatory CpG motifs

GENEART has experience in optimizing genes for all major expression systems. We have the profound knowledge to optimize and synthesize any sequence you require.

GeneOptimizer^® performance

Our GeneOptimizer^® technology has been shown to work by numerous publications. An evaluation by the University of Regensburg concluded that our gene optimization algorithm appears to outperform other state-of-the-art optimization technologies.

Human lung carcinoma cells were transiently transfected with non-optimized and optimized expression plasmids and cytokine production was quantified using commercially available ELISA assays

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