High quality plasmid DNA production
GENEART plasmid DNA purification protocols ensure consistant high quality for research applications and preclinical studies.
Benefits
- high plasmid DNA homogeneity and purity
- low levels of endotoxin (below 0.1 EU/µg pDNA, termed ‘endofree’).
- mg to gram scale
- production in shaker culture or bioreactor
- delivery options: liquid with or without cooling or frozen (dry ice)
- Fill and Finish service
Applications
- cell transfection
- immunization studies
- preclinical studies
- toxicological studies
Comprehensive quality control:
| Characteristics | Test method | Specification |
| Visual appearance | visual inspection | clear, colorless solution |
| pDNA homogeneity | AGE | ≥ 85 % supercoiled (dependent on plasmid characteristics) |
| pDNA concentration | absorption at 260 nm | provided as 0.5 – 2.0 mg/ml or as specified by customer |
| Identity | restriction digestion, AGE | co-migrates with standard |
| OD260/280 ratio | UV spectrophotometry | 1.8 - 2.0 |
| Endotoxin content | LAL assay (optional) | ≤ 0.1 EU/µg pDNA* |
AGE = agarose gel electrophoresis
*can be reduced to below 0.01 EU/µg pDNA if desired
Bioburden testing, genomic DNA and RNA assays on request
Highest quality sequencing (with Phred Scores defined by customer) available on request
Examples documentation
Take a look at the quality documentation certificates provided with any plasmid preparation order.
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Certificate of Analysis pPREP1 |
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Certificate of Analysis pP6L |
How to order
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Fill in the Excel order form and email it to plasmid@geneart.com or |
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add plasmid preparation to any other product at mygeneart.com. |
Fill & Finish service
Plasmid DNA ‘ready to use’: aliquoted and labelled according to your specifications
Simply indicate:
- concentration of DNA
- volume per aliquot
- number of aliquots desired
Service range:
- from 50 – 5,000 aliquots
- starting from 50 USD/EURO
- including tubes, labeling and packaging
- delivery options: with or without coolpacks, or frozen (dry ice)
High quality plasmid manufacturing for pre-GMP requirements
For valuable lead candidates or close-to-GMP stage projects / pre-clinical studies, GENEART manufactures plasmid DNA with technology from Boehringer Ingelheim RCV GmbH & Co KG
Benefits
- Improved product properties (enhanced immunogenicity and safety) through software assisted gene design by GENEART’s proprietary GeneOptimizer®
- Flexible access to pDNA material in consistent high quality and sufficient amounts for all phases of the development of DNA-based vaccines and therapeutics
- Identical preparation protocol for non-GMP material and subsequent cGMP manufacturing ensures perfect consistency in DNA quality
- Increased stability and production yields through rational gene design by GENEART’s proprietary GeneOptimizer®
- Regulatory authority compliance at any step
Press release Boehringer Ingelheim Austria
If you are interested in Plasmid GMP manufacturing, please call 0049-941-94276-622, or send an email to: plasmid@geneart.com; we will answer within one business day.
Our partner for plasmid GMP production:
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