Plasmid preparation
Preparation of recombinant plasmid DNA from E.coli
Amplification and purification of plasmid DNA from E. coli lysates
- low endotoxin content
- suitable for research applications such as
- transfection
- enzymatic modification
- immunization of animals
DNA is supplied on cool packs or dry ice with a quality control data sheet included.
Different amounts of plasmid DNA (1mg up to 100mg) available - please refer to our prices and production times.
Quality control includes:
| Characteristics | Test method | Specification |
| Visual appearance | visual inspection | clear, colorless solution |
| pDNA homogeneity | AGE | predominantly supercoiled |
| pDNA concentration | absorption at 260 nm | provided or as specified by customer |
| Identity | restriction digestion, AGE | co-migrates with standard |
| OD260/280 ratio | UV spectrophotometry | 1.8 - 2.0 |
| Endotoxin content | LAL assay (optional) | ≤ 0.1 EU/µg pDNA |
Sequence analysis will be provided on request.
PDF file with example of QCD for PREP1
Please use our online request form to obtain a quote for your specific project.
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