Plasmid production preclinical grade
Production of 100 mg to 1 g of plasmid DNA from E. coli batch fermentation cultures.
Plasmid DNA of reproducibly high and consistent quality for larger animal studies, preclinical tests, stability and toxicology studies.
The benefits for you
GENEART manufactures plasmid DNA using the Qiagen anion-exchange resin developed exclusively for the purification of nucleic acids. High DNA homogeneity (≥90% ccc form) and purity with low levels of endotoxin are hallmarks of this purification procedure. If necessary, further increase of plasmid ccc content and reduction of endotoxin content is possible through additional column purification steps.
Stringent quality control ensures plasmid DNA of the highest quality
Each non-GMP grade plasmid DNA lot undergoes extensive testing, and a comprehensive QC report is supplied with every plasmid DNA batch. Sterility testing and assays for E. coli genomic DNA, RNA or protein are available on request. The GENEART sequencing service can provide sequence analysis of the parent plasmid as well as the final product.
Quality control includes:
| Characteristics | Test method | Specification |
| Visual appearance | visual inspection | clear, colorless solution |
| pDNA homogenicity | AGE, oc/ccc estimate | ≥80 % ccc form (dependent on plasmid characteristics) |
| pDNA concentration | absorption at 260 nm | provided or as specified by customer |
| Identity | restriction digestion, AGE | co-migrates with standard |
| OD260/280 ratio | UV spectrophotometry | 1.8 - 2.0 |
| Endotoxin content | LAL assay (optional) | ≤ 0.1 EU/µg pDNA |
AGE = agarose gel electrophoresis
A customized service to meet the needs of your project
GENEART provides comprehensive customer service and support, starting with gene optimization and synthesis, subcloning the optimized gene(s) into a suitable vector and subsequent plasmid production in any required quantity and quality.
Plasmid vectors with GENEART's optimized gene sequences not only demonstrate improved expression and stability, but also produce increased yields in large-scale plasmid preparations.
Project planning and plasmid design for streamlined production and optimal performance is our daily business.
Please use our online request form to obtain a quote for your specific project.
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