Publications

Here are a number of selected publications of GENEART customers who have already worked successfully with GENEART's synthetic genes.

If you wish to add a specific publication to this list, please provide the reference so that it may be added. We will pay a finders fee for any new reference.

• Ludwig C, Wagner R (2007)
  Virus-like Particles - Universal Molecular Toolboxes
  Curr Opin Biotechnol 18(6):537-45
  
  
• Buguliskis J S, Casta L J, Butz C E, Matsumoto Y, Taraschi TF (2007)
  Expression and biochemical characterization of Plasmodium falciparum DNA ligase I
  Molecular and Biochemical Parasitology
  Volume 155, Issue 2, October 2007, 128-137
  
  
• Chêne et al. (2007)
  A Molecular Link between Malaria and Epstein–Barr Virus Reactivation
  PLoS Pathog 3(6): e80, June 8
  
  
• Buegl et al. (2007)
  DNA synthesis and biological security
  NATURE BIOTECHNOLOGY VOLUME 25 NUMBER 6 JUNE
  
  
• Brandt S,  Blißenbach M, Grewe B, Konietzny R, Grunwald T, Überla K (2007)
  Rev Proteins of Human and Simian Immunodeficiency Virus Enhance RNA Encapsidation
  PLoS Pathog Apr 13 2007 3(4): e54
  
  
• Poulsen TR, Meijer PJ, Jensen A, Nielsen LS, Andersen PS. (2007)
  Kinetic, Affinity, and Diversity Limits of Human Polyclonal Antibody Responses against Tetanus Toxoid
  The Journal of Immunology, 2007, 179: 3841-3850.
  
  
• Fiorucci, M, et al (2007)
  Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in hepatic injuries
  J Gen Virol 88 (2007), 1149-1162
  
  
• Gach J S, Maurer M, Hahn R, Gasser B, Mattanovich D, Katinger H, Kunert R (2007)
  High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris
  Journal of Biotechnology
  Volume 128, Issue 4, 10 March 2007, Pages 735-746
  
  
• Hernandez I, Molenaar D, Beekwilder J, Bouwmeester H,
  and van Hylckama Vlieg J E T (2007)
  Expression of Plant Flavor Genes in Lactococcus lactis
  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2007, p. 1544–1552 Vol. 73, No. 5
  
  
• Raymond C. S,  Soriano P (2007)
  High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
  PLoS ONE 2(1): e162. doi:10.1371/journal.pone.0000162
  
  
• Waterkamp D.A, Muller O.J., Ying Y, Trepel M, Kleinschmidt J.A. (2006)
  Isolation of targeted AAV2 vectors from novel virus display libraries
  J Gene Med. 2006 Nov;8 (11):1307-19
  
  
• Kong WP, Hood C, Yang ZY, Wei CJ, Xu L, Garcia-Sastre A, Tumpey TM, Nabel GJ. (2006)
  Protective immunity to lethal challenge of the 1918 pandemic influenza virus by vaccination.
  Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15987-91. Epub 2006 Oct 16
  
  
• Gomez CE, Najera JL, Jimenez EP, Jimenez V, Wagner R, Graf M, Frachette MJ, Liljestrom P, Pantaleo G, Esteban M. (2006)
  Head-to-head comparison on the immunogenicity of two HIV/AIDS vaccine candidates based on the attenuated poxvirus strains MVA and NYVAC co-expressing in a single locus the HIV-1(BX08) gp120 and HIV-1(IIIB) Gag-Pol-Nef proteins of clade B.
  Vaccine. 2006 Oct 16; [Epub ahead of print]
  
  
• Samorski R, Gissmann L and Osen W (2006)
  Codon optimized expression of HPV 16 E6 renders target cells susceptible to E6-specific CTL recognition
  Immunology Letters, Volume 107, Issue 1, 15 September 2006, Pages 41-49
  
  
• Graf M, Ludwig C, Kehlenbeck S, Jungert K, Wagner R. (2006)
  A quasi-lentiviral green fluorescent protein reporter exhibits nuclear export features of late human immunodeficiency virus type 1 transcripts
  Virology  2006 Sep 1;352(2):295-305
  
  
• Das D, Suresh M.R. (2006)
  Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene
  Journal of Virological Methods 137 Aug (2006) 343–346
  
  
• Barouch et al. (2006)
  Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
  Nature 2006 May; 441: 239-243
  
  
• Scholten K.B.J, Kramer D, Kueter E.W.M, Graf M, Schoedl T, Meijer C.J.L.M, Schreuers, M.W.J, Hooijberg, E. (2006)
  Codon modification of T cell receptores allows enhanced functional expression in transgenic human T cells
  Clin. Immunology,  2006 May;119(2):135-45.
  
  
• Mäkelä A R, Matilainen H, White D J, Ruoslahti E, and Oker-Blom C (2006)
  Enhanced Baculovirus-Mediated Transduction of Human Cancer Cells
  by Tumor-Homing Peptides
  JOURNAL OF VIROLOGY, July 2006, p. 6603–6611 Vol. 80, No. 13
  
  
• Dipti C A, Jain S K and Navin K (2006)
  A novel recombinant multiepitope protein as a hepatitis C diagnostic intermediate of high
  Protein Expression and Purification, Volume 47, Issue 1, May 2006, Pages 319-328
  
  
• Li Huijuan et al (2006)
  Optimization of humanized IgGs in glycoengineered Pichia pastoris
  Nature Biotechnology 24, 210 - 215 (2006)
  
  
• Zapf M W, Theisen S, Vogel1 R Fand Niessen L (2006)
  Cloning of Wheat LTP1500 and Two Fusarium culmorum Hydrophobins
  in Saccharomyces cerevisiae and Assessment of Their Gushing Inducing Potential in Experimental Wort Fermentation
  JOURNAL OF THE INSTITUTE OF BREWING
  VOL. 112, NO. 3, 2006
  
  
• Grimm D, Panday K, Nakai H, Storm T.A., Kay M.A. (2006)
  Liver Transduction with Recombinant Adeno-Associated Virus Is Primarily Restricted by Capsid Serotype Not Vector Genotype
  Journal of Virology, Jan. 2006, p. 426–439
  
  
• LaFayette PR, Kane PM, Phan BH, Parrott WA (2005)
  Arabitol dehydrogenase as a selectable marker for rice
  Plant Cell Reports, 2005 Dec; 24(10):596-60
  
  
• Bonamore A, Ilavi A, Giangiacomo, L, Bellelli A, Morea V, Boffi A (2005)
  A novel thermostable hemoglobin from the actinobacterium Thermobifida fosca
  FEBS Journal, 2005; 272: 4189-4201
  
  
• Wang S, Chou TH, Sakhatskyy PV, Huang S, Lawrence JM, Cao H, Huang X, Lu S. (2005)
  Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system.
  J Virol., 2005 Feb;79(3):1906-10.
  
  
• Zhong F, Cao W, Chan E, Tay PN, Cahya FF, Zhang H, Lu J. (2005)
  Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression.
  Immunology, 2005 Jan;114(1):83-93
  
  
• Karzenowski D, Potter DW, Padidam M. (2005)
  Inducible control of transgene expression with ecdysone receptor: gene switches with high sensitivity, robust expression, and reduced size.
  Biotechniques, 2005 Aug;39(2):191-6
  
  
• Steinstraesser L, Tippler B, Mertens J, Lamme E, Homann HH, Lehnhardt M, Wildner O, Steinau HU, Uberla K. (2005)
  Inhibition of early steps in the lentiviral replication cycle by cathelicidin host defense peptides.
  Retrovirology, 2005 Jan 18;2(1):2.
  
  
• van den Brink EN et al. (2005)
  Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus
  Journal of Virology, 2005, 79, 3, 1635-1644
  
  
• Nálezkováa M, de Groot A, Graf M, Gansa P, Blanchard L (2005)
  Overexpression and purification of Pyrococcus abyssi phosphopantetheine adenylyltransferase from an optimized synthetic gene for NMR studies
  Protein Expression and Purification 39, 2005
  
  
• Barouch DH et al. (2004)
  Immunogenicity of Recombinant Adenovirus Serotype 35 Vaccine in the Presence of Pre-Existing Anti-Ad5 Immunity
  The Journal of Immunology, 2004, 172: 6290-6297
  
  
• Flick K, Ahuja S, Chene A, Bejarano MT, Chen Q. (2004)
  Optimized expression of Plasmodium falciparum erythrocyte membrane protein 1 domains in Escherichia coli.
  Malar J. , 2004 Dec 15;3(1):50.
  
  
• Kuate S, Stefanou D, Hoffmann D, Wildner O, Uberla K. (2004)
  Production of lentiviral vectors by transient expression of minimal packaging genes from recombinant adenoviruses.
  J Gene Med., 2004 Nov;6(11):1197-205.
  
  
• Pollmann A, Kabisch H, Block A, Muller J, Hellwinkel OJ. (2004)
  Limited specificity of promoter constructs for gene therapy in osteosarcoma.
  Int J Mol Med., 2004 Oct;14(4):737-42.
  
  
• Young KR, Teal BE, Brooks Y, Green TD, Bower JF, Ross TM. (2004)
  Unique V3 loop sequence derived from the R2 strain of HIV-type 1 elicits broad neutralizing antibodies.
  AIDS Res Hum Retroviruses, 2004, Nov;20(11):1259-68
  
  
• Salanti A, Dahlback M, Turner L, Nielsen MA, Barfod L, Magistrado P, Jensen AT, Lavstsen T, Ofori MF, Marsh K, Hviid L, Theander TG. (2004)
  Evidence for the involvement of VAR2CSA in pregnancy-associated malaria.
  J Exp Med. , 2004 Nov 1;200(9):1197-203.
  
  
• Evans L, McMullen M, Sleep D, Waters J, Cameron J, Dodsworth N,  Tooth D and Greenfield A (2004)
  High-level secretion of bioactive scFv antibody fragments and scFv-albumin fusions by Saccharomyces cerevisiae.
  IBC's 15th Annual International Conference - Antibody Engineering , 2004,
•  Didierlaurent A et al.(2004)
  Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
  Vaccine 22, 2004, 3395-3403
  
  
• Egelhofer M et al (2004)
  Inhibition of Human Immunodeficiency Virus Type 1 Entry in Cells Expressing gp41-Derived Peptides
  Journal of Virology, 2004, 568-575
  
  
• Nessler S, Friedrich O, Bakouh N, Fink RHA, Sanchez CP, Planelles G,  Lanzer M (2004)
  Evidence for Activation of Endogenous Transporters in Xenopus laevis Oocytes Expressing the Plasmodium falciparum Chloroquine Resistance Transporter, PfCRT
  The Journal of Biological Chemistry, 2004, 279, 39438-39446
  
  
• Graf M, Deml L, Wagner R. (2004)
  Codon-optimized genes that enable increased heterologous expression in mammalian cells and elicit efficient immune responses in mice after vaccination of naked DNA.
  Methods Mol Med., 2004, 94, 197-210
  
  
• Nielsen FS, Sauer J, Backlund J, Voldborg B, Gregorius K, Mouritsen S, Bratt T (2004)
  Insertion of foreign T cell epitopes in human tumor necrosis factor alpha with minimal effect on protein structure and biological activity.
  J Biol Chem., 2004, 279 (32):33593-600
  
  
• Duan J, Antezana MA. (2003)
  Mammalian mutation pressure, synonymous codon choice, and mRNA degradation.
  J Mol Evol, 2003 Dec;57(6):694-701.
  
  
• Bradel-Tretheway BG, Zhen Z, Dewhurst S. (2003)
  Effects on codon-optimization on protein expression by the human herpesvirus 6 and 7 U51 open reading frame.
  J Virol Methods. 2003 Aug; 111(2): 145-56
  
  
• Stoll R, Renner C, Buettner R, Voelter W, Bosserhoff AK, Holak TA (2003)
  Backbone dynamics of the human MIA protein studied by (15)N NMR relaxation: implications for extended interactions of the SH3 domains.
  Protein Sci. 2003 Mar; 12(3): 510-9
  
  
• Deml L, Bojak A, Steck S, Graf M, Wild J, Schirmbeck R, Wolf H, Wagner R (2001)
  Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein.
  J Virol 2001 Nov; 75(22):10991-1001
  
  
• Wagner R, Graf M, Bieler K, Wolf H, Grunwald T, Foley P, Uberla K (2000)
  Rev-indepandant expression of synthetic gag-pol genes of immunodeficiency virus type 1and simian immunodeficiency virus: implications for the safety of lentiviral vectors.
  Hum Gene Ther 2000 Nov 20;11(17): 2403-13
  
  
• Graf M, Bolak A, Deml L, Bieler K, Wolf H, Wagner R (2000)
  Concerted action of multile cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression.
  J Virol 2000 Nov; 74(22): 10822-6