Publications
Here are a number of selected publications of GENEART customers who have already worked successfully with GENEART's synthetic genes.
If you wish to add a specific publication to this list, please provide the reference so that it may be added. We will pay a finders fee for any new reference.
- Maertens B, Spriestersbach A, von Groll U, Roth U, Kubicek J, Gerrits M, Graf M, Liss M, Daubert D, Wagner R, Schäfer F (2010)
Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli
Protein Science, Volume 19, Issue 7, pages 1312–1326, July 2010
- The MGC Project Team, Temple G, Gerhard D S, et al. (2009)
The completion of the Mammalian Gene Collection (MGC)
Genome Res. 2009 19: 2324-2333 originally published online September 18, 2009
- Gach JS, Furtmuller PG, Quendler H, Messner P, Wagner R, Katinger H, Kunert R (2009)
Proline is not uniquely capable of providing the pivot point for domain swapping in 2G12, a broadly neutralizing antibody against HIV-1.
J Biol Chem. 2009 Nov 10. [Epub ahead of print]
- Williams AJ,O'Brien LM, Phillpotts RJ, Perkins SD (2009)
Improved Efficacy of a Gene Optimised Adenovirus-based Vaccine for Venezuelan Equine Encephalitis Virus
Virology Journal 2009, 6:118, 31 July 2009
- Lloyd J, Chapman JR, Clapperton JA, Haire LF, Hartsuiker E, Li J, Carr AM, Jackson SP, Smerdon SJ (2009)
A Supramodular FHA/BRCT-Repeat Architecture Mediates Nbs1 Adaptor Function in Response to DNA Damage
Cell, Volume 139, Issue 1, 100-111, 2 October 2009. - Leiherer A, Ludwig C, Wagner R.. (2009)
Uncoupling human immunodeficiency virus type 1 Gag and Pol reading frames: role of the transframe protein p6* in viral replication.
J Virol. 2009 Jul;83(14):7210-20. Epub 2009 Apr 29.
- Scott C, Jackson, CJ, Coppin CW, Mourant RG, Hilton ME, Sutherland TD, Russell RJ and Oakeshott JG (2009)
Catalytic Improvement and Evolution of Atrazine Chlorohydrolase
Applied and Environmental Microbiology, April 2009, p. 2184-2191, Vol. 75, No. 7
- Hansen EH, Lindberg Møller B, Kock GR, Bünner CM, Kristensen C, Jensen OR, Okkels FT, Olsen CE, Motawia MS, Hansen J (2009)
De novo biosynthesis of vanillin in fission and baker's yeast
AEM Accepts, published online ahead of print on 13 March 2009
- Dubeau M-P, Ghinet MG, Jacques P-E, Clermont N, Beaulieu C, and Brzezinski R (2009)
Cytosine Deaminase as a Negative Selection Marker for Gene Disruption and Replacement in the Genus Streptomyces and Other Actinobacteria
Applied and Environmental Microbiology, February 2009, p. 1211-1214, Vol. 75, No. 4
- On C, Marshall CR., Perry SF, Le HD, Yurkov V, Omelchenko A, Hnatowich M, Hryshko LV, Tibbits GF (2009)
Characterization of zebrafish (Danio rerio) NCX4: a novel NCX with distinct electrophysiological properties
Am J Physiol Cell Physiol 296: C173-C181, 2009.
- Trollet C, Pereira Y, Burgain A, Litzler E, Mezrahi M, Seguin J, Manich M, Popoff MR, Scherman D, Bigey P (2009)
Generation of high titer neutralizing antibodies against Botulinum toxin A, B and E by DNA electrotransfer
Infect. Immun. doi:10.1128/IAI.01269-08
- Dr. Christian Kranz, Geneart AG, Regensburg, Germany (2008)
Customised vectors by gene synthesis
Euro Biotech, News Nº 9-10, Volume 7, 2008
- Dai J, Hyland EM, Yuan D, Huang H, Bader JS, Boeke JD (2008)
Probing Nucleosome Function: A Highly Versatile Library of Synthetic Histone H3 and H4 Mutants
Cell, Volume 134, Issue 6, 19 September 2008, Pages 1066-1078
- Guilligay D et al. (2008)
The structural basis for cap binding by influenza virus polymerase subunit PB2
Nat Struct Mol Biol. 2008 May;15(5):500-6. Epub 2008 May 4.
- Casta, L., J. Buguliskis, Y. Matsumoto, T. Taraschi (2008)
Expression and Biochemical Characterization of the Plasmodium falciparum DNA Repair Enzyme, Flap Endonuclease-1 (PfFEN-1).
Molecular and Biochemical Parasitology. 157:1-12.
- Zolla-Pazner S, Cohen SS, Krachmarov C, Wang S, Pinter A, Lu S. (2008)
Focusing the immune response on the V3 loop, a neutralizing epitope of the HIV-1 gp120 envelope
Virology. 2008 Mar 15;372(2):233-46. Epub 2007 Dec 3.
- Ludwig C, Wagner R (2007)
Virus-like Particles - Universal Molecular Toolboxes
Curr Opin Biotechnol 18(6):537-45
- Buguliskis J S, Casta L J, Butz C E, Matsumoto Y, Taraschi TF (2007)
Expression and biochemical characterization of Plasmodium falciparum DNA ligase I
Molecular and Biochemical Parasitology
Volume 155, Issue 2, October 2007, 128-137
- Chêne et al. (2007)
A Molecular Link between Malaria and Epstein–Barr Virus Reactivation
PLoS Pathog 3(6): e80, June 8
- Buegl et al. (2007)
DNA synthesis and biological security
NATURE BIOTECHNOLOGY VOLUME 25 NUMBER 6 JUNE
- Brandt S, Blißenbach M, Grewe B, Konietzny R, Grunwald T, Überla K (2007)
Rev Proteins of Human and Simian Immunodeficiency Virus Enhance RNA Encapsidation
PLoS Pathog Apr 13 2007 3(4): e54
- Poulsen TR, Meijer PJ, Jensen A, Nielsen LS, Andersen PS. (2007)
Kinetic, Affinity, and Diversity Limits of Human Polyclonal Antibody Responses against Tetanus Toxoid
The Journal of Immunology, 2007, 179: 3841-3850.
- Fiorucci, M, et al (2007)
Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in hepatic injuries
J Gen Virol 88 (2007), 1149-1162
- Hernandez I, Molenaar D, Beekwilder J, Bouwmeester H,
and van Hylckama Vlieg J E T (2007)
Expression of Plant Flavor Genes in Lactococcus lactis
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2007, p. 1544–1552 Vol. 73, No. 5
- Raymond C. S, Soriano P (2007)
High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
PLoS ONE 2(1): e162. doi:10.1371/journal.pone.0000162
- Waterkamp D.A, Muller O.J., Ying Y, Trepel M, Kleinschmidt J.A. (2006)
Isolation of targeted AAV2 vectors from novel virus display libraries
J Gene Med. 2006 Nov;8 (11):1307-19
- Kong WP, Hood C, Yang ZY, Wei CJ, Xu L, Garcia-Sastre A, Tumpey TM, Nabel GJ. (2006)
Protective immunity to lethal challenge of the 1918 pandemic influenza virus by vaccination.
Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15987-91. Epub 2006 Oct 16 - Samorski R, Gissmann L and Osen W (2006)
Codon optimized expression of HPV 16 E6 renders target cells susceptible to E6-specific CTL recognition
Immunology Letters, Volume 107, Issue 1, 15 September 2006, Pages 41-49
- Graf M, Ludwig C, Kehlenbeck S, Jungert K, Wagner R. (2006)
A quasi-lentiviral green fluorescent protein reporter exhibits nuclear export features of late human immunodeficiency virus type 1 transcripts
Virology 2006 Sep 1;352(2):295-305
- Das D, Suresh M.R. (2006)
Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene
Journal of Virological Methods 137 Aug (2006) 343–346 - Barouch et al. (2006)
Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
Nature 2006 May; 441: 239-243
- Scholten K.B.J, Kramer D, Kueter E.W.M, Graf M, Schoedl T, Meijer C.J.L.M, Schreuers, M.W.J, Hooijberg, E. (2006)
Codon modification of T cell receptores allows enhanced functional expression in transgenic human T cells
Clin. Immunology, 2006 May;119(2):135-45.
- Mäkelä A R, Matilainen H, White D J, Ruoslahti E, and Oker-Blom C (2006)
http://jvi.asm.org/cgi/content/full/80/13/6603?view=long&pmid=16775347Enhanced Baculovirus-Mediated Transduction of Human Cancer Cells
by Tumor-Homing Peptides
JOURNAL OF VIROLOGY, July 2006, p. 6603–6611 Vol. 80, No. 13
- Dipti C A, Jain S K and Navin K (2006)
A novel recombinant multiepitope protein as a hepatitis C diagnostic intermediate of high
Protein Expression and Purification, Volume 47, Issue 1, May 2006, Pages 319-328
- Li Huijuan et al (2006)
Optimization of humanized IgGs in glycoengineered Pichia pastoris
Nature Biotechnology 24, 210 - 215 (2006)
- Zapf M W, Theisen S, Vogel1 R Fand Niessen L (2006)
Cloning of Wheat LTP1500 and Two Fusarium culmorum Hydrophobins
in Saccharomyces cerevisiae and Assessment of Their Gushing Inducing Potential in Experimental Wort Fermentation
JOURNAL OF THE INSTITUTE OF BREWING
VOL. 112, NO. 3, 2006
- Grimm D, Panday K, Nakai H, Storm T.A., Kay M.A. (2006)
Liver Transduction with Recombinant Adeno-Associated Virus Is Primarily Restricted by Capsid Serotype Not Vector Genotype
Journal of Virology, Jan. 2006, p. 426–439
- LaFayette PR, Kane PM, Phan BH, Parrott WA (2005)
Arabitol dehydrogenase as a selectable marker for rice
Plant Cell Reports, 2005 Dec; 24(10):596-60
- Bonamore A, Ilavi A, Giangiacomo, L, Bellelli A, Morea V, Boffi A (2005)
A novel thermostable hemoglobin from the actinobacterium Thermobifida fosca
FEBS Journal, 2005; 272: 4189-4201
- Wang S, Chou TH, Sakhatskyy PV, Huang S, Lawrence JM, Cao H, Huang X, Lu S. (2005)
Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system.
J Virol., 2005 Feb;79(3):1906-10.
- Zhong F, Cao W, Chan E, Tay PN, Cahya FF, Zhang H, Lu J. (2005)
Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression.
Immunology, 2005 Jan;114(1):83-93
- Karzenowski D, Potter DW, Padidam M. (2005)
Inducible control of transgene expression with ecdysone receptor: gene switches with high sensitivity, robust expression, and reduced size.
Biotechniques, 2005 Aug;39(2):191-6
- Steinstraesser L, Tippler B, Mertens J, Lamme E, Homann HH, Lehnhardt M, Wildner O, Steinau HU, Uberla K. (2005)
Inhibition of early steps in the lentiviral replication cycle by cathelicidin host defense peptides.
Retrovirology, 2005 Jan 18;2(1):2.
- van den Brink EN et al. (2005)
Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus
Journal of Virology, 2005, 79, 3, 1635-1644
- Nálezkováa M, de Groot A, Graf M, Gansa P, Blanchard L (2005)
Overexpression and purification of Pyrococcus abyssi phosphopantetheine adenylyltransferase from an optimized synthetic gene for NMR studies
Protein Expression and Purification 39, 2005
- Barouch DH et al. (2004)
Immunogenicity of Recombinant Adenovirus Serotype 35 Vaccine in the Presence of Pre-Existing Anti-Ad5 Immunity
The Journal of Immunology, 2004, 172: 6290-6297
- Flick K, Ahuja S, Chene A, Bejarano MT, Chen Q. (2004)
Optimized expression of Plasmodium falciparum erythrocyte membrane protein 1 domains in Escherichia coli.
Malar J. , 2004 Dec 15;3(1):50.
- Kuate S, Stefanou D, Hoffmann D, Wildner O, Uberla K. (2004)
Production of lentiviral vectors by transient expression of minimal packaging genes from recombinant adenoviruses.
J Gene Med., 2004 Nov;6(11):1197-205.
- Pollmann A, Kabisch H, Block A, Muller J, Hellwinkel OJ. (2004)
Limited specificity of promoter constructs for gene therapy in osteosarcoma.
Int J Mol Med., 2004 Oct;14(4):737-42.
- Young KR, Teal BE, Brooks Y, Green TD, Bower JF, Ross TM. (2004)
Unique V3 loop sequence derived from the R2 strain of HIV-type 1 elicits broad neutralizing antibodies.
AIDS Res Hum Retroviruses, 2004, Nov;20(11):1259-68
- Salanti A, Dahlback M, Turner L, Nielsen MA, Barfod L, Magistrado P, Jensen AT, Lavstsen T, Ofori MF, Marsh K, Hviid L, Theander TG. (2004)
Evidence for the involvement of VAR2CSA in pregnancy-associated malaria.
J Exp Med. , 2004 Nov 1;200(9):1197-203.
- Evans L, McMullen M, Sleep D, Waters J, Cameron J, Dodsworth N, Tooth D and Greenfield A (2004)
High-level secretion of bioactive scFv antibody fragments and scFv-albumin fusions by Saccharomyces cerevisiae.
IBC's 15th Annual International Conference - Antibody Engineering , 2004,
- Nessler S, Friedrich O, Bakouh N, Fink RHA, Sanchez CP, Planelles G, Lanzer M (2004)
Evidence for Activation of Endogenous Transporters in Xenopus laevis Oocytes Expressing the Plasmodium falciparum Chloroquine Resistance Transporter, PfCRT
The Journal of Biological Chemistry, 2004, 279, 39438-39446
- Graf M, Deml L, Wagner R. (2004)
Codon-optimized genes that enable increased heterologous expression in mammalian cells and elicit efficient immune responses in mice after vaccination of naked DNA.
Methods Mol Med., 2004, 94, 197-210
- Nielsen FS, Sauer J, Backlund J, Voldborg B, Gregorius K, Mouritsen S, Bratt T (2004)
Insertion of foreign T cell epitopes in human tumor necrosis factor alpha with minimal effect on protein structure and biological activity.
J Biol Chem., 2004, 279 (32):33593-600
- Duan J, Antezana MA. (2003)
Mammalian mutation pressure, synonymous codon choice, and mRNA degradation.
J Mol Evol, 2003 Dec;57(6):694-701.
- Bradel-Tretheway BG, Zhen Z, Dewhurst S. (2003)
Effects on codon-optimization on protein expression by the human herpesvirus 6 and 7 U51 open reading frame.
J Virol Methods. 2003 Aug; 111(2): 145-56
- Stoll R, Renner C, Buettner R, Voelter W, Bosserhoff AK, Holak TA (2003)
Backbone dynamics of the human MIA protein studied by (15)N NMR relaxation: implications for extended interactions of the SH3 domains.
Protein Sci. 2003 Mar; 12(3): 510-9
Vaccine Development
- Ramanathand MP, Kutzlerc MA, Kuoa Y-C, Yana J, Liua H, Shaha V, Bawaa A, Sellingb B, Sardesaid NY, Kimd JJ and Weiner DB (2009)
Coimmunization with an optimized IL15 plasmid adjuvant enhances humoral immunity via stimulating B cells induced by genetically engineered DNA vaccines expressing consensus JEV and WNV E DIII
Vaccine, Article in Press
- Mooij P et al. (2009)
Comparison of human and rhesus macaque T-cell responses elicited by boosting with NYVAC encoding HIV-1 clade C immunogens
J. Virol. doi:10.1128/JVI.02345-08
- Simabucoa FM, Tamuraa RE, Carromeua C, Farinha-Arcieria LE, Ventura AM (2009)
Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice
Journal of Virological Methods, Article in Press
- Dasa D, Mongkolaungkoona S, and Suresh MR (2009)
Super induction of dengue virus NS1 protein in E. coli
doi:10.1016/j.pep.2009.02.003
- LADDY DJ, YAN J, KUTZLER M, KOBASA D, KOBINGER GP, KHAN AS, GREENHOUSE J, SARDESAI NY, DRAGHIA-AKLI R, WEINER DB. (2008)
Heterosubtypic Protection against Pathogenic Human
and Avian Influenza Viruses via In Vivo Electroporation
of Synthetic Consensus DNA Antigens
PLoS ONE. 2008 Jun 25;3(6):e2517
- SPETH C, BREDL S, HAGLEITNER M, WILD J, DIERICH M, WOLF H, SCHROEDER J, WAGNER R, DEML L. (2008)
Human immunodeficiency virus type-1 (HIV-1) Pr55(gag) virus-like particles are potent activators of human monocytes.
Virology, Epub ahead of print
- BART PA, GOODALL R, BARBER T, HARARI A, GUIMARAES-WALKER A, KHONKARLY M, SHEPPERD N, BANGALA Y, FRACHETTE MJ, WAGNER R, LILJESTROM P, KRAHENBUHL JP, GIRARD M, GOUDSCHMIDT J, ESTEBAN M, HEENEY J, SATTENTAU Q, MCCORMACK S, BABIKER A, PANTALEO G, WEBER J. (2008)
EV01: A Phase I trial in healthy HIV negative volunteers to evaluate a clade C HIV vaccine, NYVAC-C undertaken by the EuroVacc Consortium.
Vaccine 13;26(25):3153-61; 2008
- MCCORMACK S, STÖHR W, BARBER T, BART PA, HARARI A, MOOG CH, CIUFFREDA D, CELLERAI CH, COWEN M, GAMBONI R, BURNET S, LEGG K, BRODNICKI E, WOLF H, WAGNER R, HEENEY J, FRACHETTE MJ, TARTAGLIA J, BABIKER A, PANTALEO G, WEBER J. (2008)
EV02: A Phase I trial to compare the safety and immunogenicity of HIV DNA-C prime-NYVAC-C boost to NYVAC-C alone.
Vaccine 13;26(25):3162-74; 2008
- VERSTREPEN BE, BINS AD, ROLLIER CS, MOOIJ P, KOOPMAN G, SHEPPARD NC, SATTENTAU Q, WAGNER R, WOLF H, SCHUMACHER TN, HEENEY JL, HAANEN JB. (2008)
Improved HIV-1 specific T-cell responses by short-interval DNA tattooing as compared to intramuscular immunization in non-human primates.
Vaccine 26(26):3346-51; 2008
- HAMMER D, WILD J, LUDWIG C, ASBACH B, NOTKA F, WAGNER R. (2008)
Fusion of EBNA1-Derived Gly-Ala Repeat to Transdominant HIV-1 Gag Increases Inhibitory Activities and Survival of Transduced Cells In Vivo.
Hum Gene Ther 19(6):622-34; 2008
- STOLTE-LEEB N, BIELER K, KÖSTLER J, HEENEY J, TEN HAAFT P, SUH Y, HUNSMANN G, STAHL-HENNIG C, WAGNER R. (2008)
Better Protective Effects in Rhesus Macaques by Combining Systemic and Mucosal Application of a Dual Component Vector Vaccine After Rectal SHIV89.6P Challenge Compared to Systemic Vaccination Alone.
Viral Immunol 21(2):235-46; 2008
- LUDWIG C, LEIHERER A, WAGNER R. (2008)
Importance of protease cleavage sites within and flanking HIV-1 transframe protein p6* for spatiotemporal regulation of protease activation.
J Virol 82(9):4573-84; 2008
- CORBETT M, BOGERS W, HEENEY JL, GERBER S, GENIN C, DIDIERLAURENT A, OOSTERMEIJER H, DUBBES R, BRASKAMP G, LERONDEL S, GOMEZ CE, ESTEBAN M, WAGNER R, KONDOVA I, MOOIJ P, BALLA-JHAGJHOORSINGH J, BEENHAKKER N, KOOPMAN G, VAN DER BURG S, KRAEHENBUHL JP, LE PAPE A. (2008)
Aerosol immunization with NYVAC and MVA vectored vaccines is safe, simple, and immunogenic.
Proc Natl Acad Sci USA 105(6):2046-51; 2008
- HARARI A, BART PA, STÖHR W, TAPIA G, GARCIA M, MEDJITNA-RAIS E, BURNET S, CELLERAI C, ERLWEIN O, BARBER T, MOOG C, LILJESTROM P, WAGNER R, WOLF H, KRAEHENBUHL JP, ESTEBAN M, HEENEY J, FRACHETTE MJ, TARTAGLIA J, MCCORMACK S, BABIKER A, WEBER J, PANTALEO G. (2008)
An HIV-1 clade C DNA prime, NYVAC boost vaccine regimen induces reliable, polyfunctional, and long-lasting T cell responses.
J Exp Med 205(1):63-77; 2008
- MOOIJ P, BALLA-JHAGJHOORSINGH SS, KOOPMAN G, BEENHAKKER N, VAN HAAFTEN P, BAAK I, NIEUWENHUIS I, KONDOVA I, WAGNER R, WOLF H, GÓMEZ CE, NÁJERA JL, JIMÉNEZ V, ESTEBAN M, HEENEY JL. (2008)
Differential CD4+ versus CD8+ T-cell responses elicited by different Poxvirus-based HIV-1 vaccine candidates provide comparable efficacy in primates.
J Virol 82(6):2975-88; 2008
- GOLDWICH A, HAHN SSC, SCHREIBER S, MEIER S, KÄMPGEN E, WAGNER R, LUTZ M, SCHUBERT U. (2008)
Targeting HIV-1 Gag into the Defective Ribosomal Product Pathway Enhances MHC Class I Antigen Presentation and CD8+ T Cell Activation.
J Immunol 180(1):372-82; 2008
- Gach J S, Maurer M, Hahn R, Gasser B, Mattanovich D, Katinger H, Kunert R (2007)
High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris
Journal of Biotechnology
Volume 128, Issue 4, 10 March 2007, Pages 735-746
- Gomez CE, Najera JL, Jimenez EP, Jimenez V, Wagner R, Graf M, Frachette MJ, Liljestrom P, Pantaleo G, Esteban M. (2006)
Head-to-head comparison on the immunogenicity of two HIV/AIDS vaccine candidates based on the attenuated poxvirus strains MVA and NYVAC co-expressing in a single locus the HIV-1(BX08) gp120 and HIV-1(IIIB) Gag-Pol-Nef proteins of clade B.
Vaccine. 2006 Oct 16;
- Didierlaurent A et al.(2004)
Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
Vaccine 22, 2004, 3395-3403
- Egelhofer M et al (2004)
Inhibition of Human Immunodeficiency Virus Type 1 Entry in Cells Expressing gp41-Derived Peptides
Journal of Virology, 2004, 568-575
- Deml L, Bojak A, Steck S, Graf M, Wild J, Schirmbeck R, Wolf H, Wagner R (2001)
Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein.
J Virol 2001 Nov; 75(22):10991-1001
- Wagner R, Graf M, Bieler K, Wolf H, Grunwald T, Foley P, Uberla K (2000)
Rev-independent expression of synthetic gag-pol genes of immunodeficiency virus type 1and simian immunodeficiency virus: implications for the safety of lentiviral vectors.
Hum Gene Ther 2000 Nov 20;11(17): 2403-13
- Graf M, Bolak A, Deml L, Bieler K, Wolf H, Wagner R (2000)
Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression.
J Virol 2000 Nov; 74(22): 10822-6



